Perfluoroalkyl substances are widely used anthropogenic compounds with many structures and applications. Over the years, there has been a great concern regarding their usage as exposure can result in liver toxicity, endocrine disruption and bioaccumulation. Due to these concerns PFOS and PFOA have been banned, thus making short-chained PFAS more prevalent within the market. Due to this, the presence of short-chain PFAS within commercial products and within the environment has risen. Current research focuses on legacy long-chained PFAS, with very little information available for the health and environmental risks of short-chain PFAS. Therefore, more information on short-chained PFAS toxicity is required as their prevalence increases. To determine the toxicity of short-chain PFAS in comparison to its’ long-chained counterparts, fathead minnow (Promelas pimephales) were exposed to PFOS (5ug/L), PFHxS (5ug/L), PFBS(5ug/L) as well as an environmentally relevant mixture (PFOS: 110ng/L, PFHxS:10ng/L, PFBS: 20ng/L) for 28 days. Post exposure, mucus, blood and brain were collected and stored at -80oC for future proteomic analysis. Muscle was also collected to determine bioaccumulation. Proteins from samples were digested with heat and formic acid, and analyzed with a LC-QTOF (Agilent 1260 LC and 6545 QTOF) using data-dependent acquisition (DDA) to create a spectral peptide library, and then MS1 filtering was performed using Skyline software and their DDA workflow. In this presentation, proteomic data will be presented, with emphasis on impacts to cellular function, and potential disease outcomes.